Produced from sera of goats pre-immunized with highly pure recombinant Human SCGF-beta. Anti-Human SCGF-beta specific antibody was purified by affinity chromatography employing immobilized Human SCGF-beta matrix.
Formulierung:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Anwendungsbeschreibung:
WesternBlot: To detect Human SCGF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SCGF-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect Human SCGF-beta by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human SCGF-beta (60-293BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SCGF-beta.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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