Produced in mice using highly pure recombinant Human soluble IL-2 Receptor alpha as the immunizing antigen. This IgG2aK antibody was purified from cell culture by Protein G affinity chromatography.
Formulierung:
A sterile filtered antibody solution was lyophilized from PBS.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
WesternBlot: To detect Human sIL-2Ralpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sIL-2Ralpha<span style=line-height: 115%, font-family: times new roman, serif, font-size: 12pt,> is 2.0-4.0 ng/lane, under non-reducing conditions.. Sandwich: In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 100 pg/ml of recombinant Human sIL-2Ralpha when used in conjunction with compatible secondary reagents.. Immunohistochemistry: This antibody stained frozen human tonsil. The recommended concentration is 5.0 µg/ml at room temperature. A labeled polymer detection system was used with DAB chromogen. Optimal concentrations and conditions may vary.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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