| D-Luciferin potassium salt is the natural specific substrate for firefly luciferase (FLuc) and one of the most commonly used dyes in bioluminescence and in vivo imaging experiments. D-Luciferin potassium salt is catalysed by FLuc into luciferin in the presence of ATP, magnesium ions, and oxygen, emitting fluorescence with a peak wavelength of approximately 560 nm. In experiments, by integrating the FLuc gene as a reporter element into target cells (such as tumour cells, stem cells, or pathogenic microorganisms) or transgenic animals, followed by intraperitoneal or intravenous injection of D-luciferin potassium salt, real-time, non-invasive tracking and quantitative analysis of these targets can be achieved using imaging equipment. |
