Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
Immunogen:
The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2E3
Alternative Synonym:
UBCH9, UbcM2
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Lanes: 1: 40ng HIS-UBE2D1 protein 2: 40ng HIS-UBE2D2 protein 3: 40ng HIS-UBE2D3 protein 4: 40ng HIS-UBE2D4 protein 5: 40ng HIS-UBE2E1 protein 6: 40ng HIS-UBE2E2 protein 7: 40ng HIS-UBE2E3 protein 8: 40ng HIS-UBE2K protein 9: 40ng HIS-UBE2L3 protein 10: 40ng HIS-UBE2N protein 11: 40ng HIS-UBE2V1 protein 12: 40ng HIS-UBE2V2 protein. Primary Antibody Dilution: 1:500 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1:50,000 Gene Name: UBE2E3 Submitted by: Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.
UBE2E3 antibody - N-terminal region (ARP43070_P050) in Human HT1080 using Western Blot
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