A synthetic peptide corresponding to a sequence at the N-terminus of human CHRNA5, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.
Alternative Synonym:
CHRNA5, LNCR2, NACHRA5
Boster Bio Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Antibody Picoband catalog A02359-2. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Klonalität:
Polyclonal
Konzentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat Western blot, 0.1-0.5µg/ml, Human, RatImmunohistochemistry (Frozen Section), 0.5-1µg/ml, Human Immunocytochemistry, 0.5-1µg/ml, Human Flow Cytometry (Fixed), 1-3µg/1x106 cells
IHC analysis of CHRNA5 using anti-CHRNA5 antibody (A02359-2). CHRNA5 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-FBXL4 Antibody (A02359-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of CHRNA5 using anti-CHRNA5 antibody (A02359-2). CHRNA5 was detected in paraffin-embedded section of mouse cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CHRNA5 Antibody (A02359-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of CHRNA5 using anti-CHRNA5 antibody (A02359-2). CHRNA5 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CHRNA5 Antibody (A02359-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of CHRNA5 using anti-CHRNA5 antibody (A02359-2). CHRNA5 was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-FBXL4 Antibody (A02359-2) overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog SA1022) with DAB as the chromogen.
IHC analysis of CHRNA5 using anti-CHRNA5 antibody (A02359-2). CHRNA5 was detected in paraffin-embedded section of human prostatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&44, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissu
Western blot analysis of CHRNA5 using anti-CHRNA5 antibody (A02359-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat skeletal muscle tissue lysates&44, lane 2: HEPG2 whole cell lysates. After Electrophoresis&44, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRNA5 antigen affinity purified polyclonal antibody (Catalog A02359-2) at 0.5 µg/mL overnight at 4C&44, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog EK1002) with Tanon 5200 system. A specific band was detected for CHRNA5 at approximately 53KD. The expected band size for CHRNA5 is at 53KD.
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