Phospho-Histone H1.4 (Thr18) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB100267
- Bilder (6)
| Artikelname: | Phospho-Histone H1.4 (Thr18) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB100267 |
| Hersteller Artikelnummer: | orb100267 |
| Alternativnummer: | BYT-ORB100267-50,BYT-ORB100267-100,BYT-ORB100267-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, IF, IHC-Fr, IHC-P |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human Histone H1.4 around the phosphorylation site of Thr18 EK(p-T)P |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H1-4 | Histone H1.4 (p-T18), p-Histone H1.4, phospho-Histone H1.4, H1.4T18P, p-H1.4, H1.4, H1E, H1F4, H1s-4, HIST1H1E, RMNS, dJ221C16.5, H1 VAR.2, H1-4, H1var2, H14_HUMAN, Histone H1b, Histone H1s-4, H14_MOUSE, |
| Phospho-Histone H1.4 (Thr18) Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 22 kDa |
| UniProt: | P10412 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | H1-4 |
| Application Verdünnung: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-phospho-Histone H1.4 (Thr18) antibody (orb100267), Dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Histone H1.4 (Thr18)) Polyclonal Antibody, Unconjugated (orb100267) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Histone H1.4 (Thr18)) Polyclonal Antibody, Unconjugated (orb100267) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Histone H1.4 (Thr18)) Polyclonal Antibody, Unconjugated (orb100267) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Histone H1.4 (Thr18)) Polyclonal Antibody, Unconjugated (orb100267) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-Histone H1.4 (Thr18) Polyclonal Antibody, Unconjugated (orb100267) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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