Gamma-Adaptin Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB10106
- Bilder (7)
| Artikelname: | Gamma-Adaptin Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB10106 |
| Hersteller Artikelnummer: | orb10106 |
| Alternativnummer: | BYT-ORB10106-50,BYT-ORB10106-100,BYT-ORB10106-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Gamma-Adaptin (721-822/822aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | AP1G1_HUMAN, AP1G1, Adaptor protein complex AP-1 subunit gamma-1, Adaptor-related protein complex 1 subunit gamma-1, Clathrin assembly protein complex 1 gamma-1 large chain, Gamma1-adaptin, Golgi adaptor HA1/AP1 adaptin subunit gamma-1, ADTG, CLAPG1, AP1G1_MOUSE, Gamma-adaptin, |
| Gamma-Adaptin Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 91 kDa |
| UniProt: | O43747 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | AP1G1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
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Cell culture (MCF7), Paraformaldehyde-fixed, Incubated by 0.3% Triton-100 for 20 min, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (orb10106) at 1:200 overnight at 4C, followed by a conjugated secondary antibody (orb868805) for 90 minutes and DAPI staining. |
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Cell culture (SW480), Paraformaldehyde-fixed, Incubated by 0.3% Triton-100 for 20 min, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (orb10106) at 1:200 overnight at 4C, followed by a conjugated secondary antibody (orb868805) for 90 minutes and DAPI staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (orb10106) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: 293T (Human) Cell Lysate at 30 ug, Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-Gamma-Adaptin (orb10106) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 91 kD, Observed band size: 91 kD. |
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Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated (orb10106) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/cell: Rat brian, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Gamma-Adaptin, Unconjugated (orb10106) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/cell: rat lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated (orb10106) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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