MMP-1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB101432
- Bilder (7)
| Artikelname: | MMP-1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB101432 |
| Hersteller Artikelnummer: | orb101432 |
| Alternativnummer: | BYT-ORB101432-50,BYT-ORB101432-100,BYT-ORB101432-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human MMP-1 (251-350/469aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | CLG, CLGN, MMP-13, Mmp1, Mmp1a, MMP-1, MMP1_BOVIN, Fibroblast collagenase, Matrix metalloproteinase-1 (MMP-1), 3.4.24.7, MMP1_HUMAN, MMP13_MOUSE, Mmp13, Matrix metalloproteinase-13 (MMP-13), 3.4.24.-, MMP1_PIG, MMP1_RABIT, |
| MMP-1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 27/41/54 kDa |
| UniProt: | P03956 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | MMP1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
|
|
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MMP-1) Polyclonal Antibody, Unconjugated (orb101432) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MMP-1) Polyclonal Antibody, Unconjugated (orb101432) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MMP-1) Polyclonal Antibody, Unconjugated (orb101432) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MMP-1) Polyclonal Antibody, Unconjugated (orb101432) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MMP-1) Polyclonal Antibody, Unconjugated (orb101432) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MMP-1) Polyclonal Antibody, Unconjugated (orb101432) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Sample: Lane 1: Testis (Rat) Lysate at 40 ug, Lane 2: Testis (Mouse) Lysate at 40 ug, Primary: Anti-MMP-1 (orb101432) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD. |
Produktgarantie und fachkundiger Support
