HRG beta 1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB101689
- Bilder (7)
| Artikelname: | HRG beta 1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB101689 |
| Hersteller Artikelnummer: | orb101689 |
| Alternativnummer: | BYT-ORB101689-50,BYT-ORB101689-100,BYT-ORB101689-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Neuregulin 1 (65-150/640aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, MST131, MSTP131, NDF, NRG1-IT2, SMDF, 6030402G23Rik, D230005F13Rik, GGFII, HRGalpha, Pro-NRG1, NRG1_HUMAN, NRG1, |
| HRG beta 1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 70 kDa |
| UniProt: | Q02297 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | NRG1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:200-800 |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HRG beta 1) Polyclonal Antibody, Unconjugated (orb101689) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HRG beta 1) Polyclonal Antibody, Unconjugated (orb101689) at 1:400 overnight at 4C, followed by a conjugated secondary antibody (orb868805) for 90 minutes, and DAPI for nuclei staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HRG beta 1) Polyclonal Antibody, Unconjugated (orb101689) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Brain (Mouse) Lysate at 40 ug, Raji Cell (Human) Lysate at 40 ug, Primary: Anti-HRG beta 1 (orb101689) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 70 kD, Observed band size: 65 kD. |
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Tissue/cell: MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (HRG beta 1) Polyclonal Antibody, Unconjugated (orb101689) 1:200, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) at 37C for 90 minutes, DAPI (5 ug/ml, blue) was used to stain the cell nuclei. |
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Tissue/cell: Mouse ovary tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-HRG beta 1 Polyclonal Antibody, Unconjugated (orb101689) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/cell: U251 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (HRG beta 1) Polyclonal Antibody, Unconjugated (orb101689) 1:200, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) at 37C for 90 minutes, DAPI (5 ug/ml, blue) was used to stain the cell nuclei. |
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