MMP-2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB101824
- Bilder (6)
| Artikelname: | MMP-2 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB101824 |
| Hersteller Artikelnummer: | orb101824 |
| Alternativnummer: | BYT-ORB101824-50,BYT-ORB101824-100,BYT-ORB101824-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | KLH conjugated synthetic peptide derived from human MMP-2 (401-500/662aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1, MMP2_HUMAN, MMP2, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2 (MMP-2), 3.4.24.24, |
| MMP-2 Rabbit Polyclonal Antibody |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
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Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-MMP2 antibody (orb101824), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/test. |
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Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MMP-2) Polyclonal Antibody, Unconjugated (orb101824) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MMP-2) Polyclonal Antibody, Unconjugated (orb101824) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: A431 (Human) Cell Lysate at 30 ug, Mcf-7 (Human) Cell Lysate at 30 ug, Primary: Anti-MMP-2 (orb101824) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD. |
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Sample: SY5Y (Human) Cell Lysate at 30 ug, U2os (Human) Cell Lysate at 30 ug, U251 (Human) Cell Lysate at 30 ug, Siha (Human) Cell Lysate at 30 ug, Primary: Anti-MMP-2 (orb101824) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 60 kD. |
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Tissue/cell: U-87MG cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (MMP2) polyclonal Antibody, Unconjugated (orb101824) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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