CAMK2A + CAMK2B + CAMK2D Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB10224
- Bilder (7)
| Artikelname: | CAMK2A + CAMK2B + CAMK2D Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB10224 |
| Hersteller Artikelnummer: | orb10224 |
| Alternativnummer: | BYT-ORB10224-50,BYT-ORB10224-100,BYT-ORB10224-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ICC, IF, IHC-Fr, IHC-P |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human CaMK2 beta/ gamma/ delta (1-100/479aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | CAMKA, CaMKIINalpha, CaMKIIalpha, MRD53, MRT63, CAM2, CAMK2, CAMKB, CaMKIIbeta, MRD54, CAMKD, CAMK, CAMK-II, CAMKG, MRD59, 2810011D23Rik, 8030469K03Rik, CaMK II, [d]-CaMKII, CaMKII, mKIAA0968, Ck2b, CAMK1, Camki, RATCAMKI, PK2CDD, PKCCD, KCC2D_BOVIN, CAMK2D, CaM kinase II subunit delta, CaMK-II subunit delta, 2.7.11.17, KCC2B_BOVIN, CAMK2B, CaM kinase II subunit beta, CaMK-II subunit beta, KCC2B_HUMAN, KCC2G_HUMAN, CAMK2G, CaM kinase II subunit gamma, CaMK-II subunit gamma, KCC2D_HUMAN, KCC2A_HUMAN, CAMK2A, CaM kinase II subunit alpha, CaMK-II subunit alpha, KIAA0968, KCC2A_MOUSE, KCC2B_MOUSE, KCC2D_MOUSE, Kiaa4163, KCC2G_MOUSE, KCC2B_RAT, KCC2A_RAT, KCC2G_RAT, KCC2D_RAT, |
| CAMK2A + CAMK2B + CAMK2D Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 53-73 kDa |
| UniProt: | Q13554 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | CAMK2A |
| Application Verdünnung: | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
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4% Paraformaldehyde-fixed SH-SY5Y (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (CAMK2A + CAMK2B + CAMK2D) polyclonal Antibody, unconjugated (orb10224) 1:50, 90 min at 37C, followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control. |
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Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CAMK2A + CAMK2B + CAMK2D) Polyclonal Antibody, Unconjugated (orb10224) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CAMK2A + CAMK2B + CAMK2D) Polyclonal Antibody, Unconjugated (orb10224) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CaMK2b) Polyclonal Antibody, Unconjugated (orb10224) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CaMK2b) Polyclonal Antibody, Unconjugated (orb10224) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-CAMK2A+CAMK2B+CAMK2D (orb10224) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 53-73 kD, Observed band size: 60 kD. |
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Sample: Small intestine (Mouse) Lysate at 40 ug, Primary: Anti-CAMK2A+CAMK2B+CAMK2D (orb10224) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 53-73 kD, Observed band size: 60 kD. |
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