Caveolin-1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB10244
- Bilder (6)
| Artikelname: | Caveolin-1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB10244 |
| Hersteller Artikelnummer: | orb10244 |
| Alternativnummer: | BYT-ORB10244-50,BYT-ORB10244-100,BYT-ORB10244-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Caveolin-1 (2-120/178aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | BSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21, Cav, Cav-1, CAV1_CANLF, CAV1, Vesicular integral-membrane protein VIP21, CAV1_HUMAN, CAV1_MOUSE, CAV1_RAT, |
| Caveolin-1 Rabbit Polyclonal Antibody |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Caveolin-1) Polyclonal Antibody, Unconjugated (orb10244) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining. |
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Sample: Lung (Mouse) Lysate at 40 ug, Bladder (Mouse) Lysate at 40 ug, Primary: Anti-Caveolin-1 (orb10244) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kD, Observed band size: 20 kD. |
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Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-Caveolin-1 (orb10244) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kD, Observed band size: 20 kD. |
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Sample: NIH/3T3 Cell Lysate at 40 ug, A549 Cell Lysate at 40 ug, HUVEC Cell Lysate at 40 ug, Primary: Anti-Caveolin-1 (orb10244) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kD, Observed band size: 20 kD. |
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Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Caveolin-1 Polyclonal Antibody, Unconjugated (orb10244) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/cell: mouse retina tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Caveolin-1 Polyclonal Antibody, Unconjugated (orb10244) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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