CDK1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB10355
- Bilder (7)
| Artikelname: | CDK1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB10355 |
| Hersteller Artikelnummer: | orb10355 |
| Alternativnummer: | BYT-ORB10355-50,BYT-ORB10355-100,BYT-ORB10355-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human CDK1 (201-297/297aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | CDC2, CDC28A, P34CDC2, Cdc2a, p34, CDK1_CHICK, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, 2.7.11.22, 2.7.11.23, CDK1_HUMAN, CDKN1, CDK1_MOUSE, CDK1_RAT, |
| CDK1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 34 kDa |
| UniProt: | P06493 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | CDK1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (CDK1) polyclonal Antibody, Unconjugated (orb10355) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CDK1) Polyclonal Antibody, Unconjugated (orb10355) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CDK1) Polyclonal Antibody, Unconjugated (orb10355) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-CDK1 (orb10355) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 33 kD. |
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Sample: U251 (Human) Cell Lysate at 30 ug, A673 (Human) Cell Lysate at 30 ug, MCF-7 (Human) Cell Lysate at 30 ug, MDA-MB-231 (Human) Cell Lysate at 30 ug, Primary: Anti-CDK1 (orb10355) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD. |
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Tissue/Cell: human breast carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CDK1 Polyclonal Antibody, Unconjugated (orb10355) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: human laryngocarcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CDK1 Polyclonal Antibody, Unconjugated (orb10355) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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