DVL1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB10561
- Bilder (6)
| Artikelname: | DVL1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB10561 |
| Hersteller Artikelnummer: | orb10561 |
| Alternativnummer: | BYT-ORB10561-50,BYT-ORB10561-100,BYT-ORB10561-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human DVL1 (21-100/695aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | DVL1_PANTR, DVL1, Dishevelled-1, DSH homolog 1, DVL1_HUMAN, DVL1_MOUSE, Dvl, DVL1_RAT, |
| DVL1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 76 kDa |
| UniProt: | O14640 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | DVL1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DVL1) Polyclonal Antibody, Unconjugated (orb10561) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DVL1) Polyclonal Antibody, Unconjugated (orb10561) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Protein: Brain (Rat) lysate at 30 ug, Lung (Rat) lysate at 30 ug, Primary: Anti-DVL1/Dishevelled (orb10561) at 1:200 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 76kD, Observed band size: 76kD. |
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Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human Hela cell lysates, Primary: Anti-DVL1 (orb10561) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 76 kDa, Observed band size: 75 kDa. |
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Sample: Testis (Mouse) Lysate at 40 ug, NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-DVL1 (orb10561) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 76 kD, Observed band size: 77 kD. |
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Tissue/Cell: human cervical carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-DVL1/Dishevelled Polyclonal Antibody, Unconjugated (orb10561) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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