Phospho-Histone H3 (Ser10) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB106186
- Bilder (6)
| Artikelname: | Phospho-Histone H3 (Ser10) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB106186 |
| Hersteller Artikelnummer: | orb106186 |
| Alternativnummer: | BYT-ORB106186-50,BYT-ORB106186-100,BYT-ORB106186-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, IF, IHC-Fr, IHC-P |
| Spezies Reaktivität: | Human, Rat |
| Immunogen: | KLH conjugated synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser10 RK(p-S)TG |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H3C1 | Histone H3 (p-S10), p-Histone H3, phospho-Histone H3, H3S10P, p-H3, H3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, H3/b, H3C1, H3FB, HIST1H3D, H3.1, H3/c, H3FC, HIST1H3C, H3/d, H3FD, HIST1H3E, H3.f, H3/f, H3FF, HIST1H3I, H3/h, H3FH, HIST1H3G, H3/j, H3FJ, HIST1H3J, H3/k, H3F1K, H3FK, HIST1H3H, H3/l, H3FL, HIST1H3B, H3/i, H3FI, HIST1H3F, H3.2-221, H3c13, H3c14, H3c15, Hist1, H3-143, H3-b, H3-53, H3-f, H3-291, H3-H, H3.1-221, H3 histone, Hist1h2ail, Hist1h2ail1, Hist2h3c, Hist3h3, H31_HUMAN, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FC HIST1H3C, H33_HUMAN, H3-3A, H3-3B, H3.3A, H3F3, H3F3A, H3.3B, H3F3B, H31T_HUMAN, H3-4, H3/t, H3t, H3/g, Histone H3.4, H3FT, H3C_HUMAN, H3-5, Histone H3.5, H3F3C, H32_HUMAN, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, Histone H3/m, Histone H3/o, HIST2H3A, H3F2, H3FM, HIST2H3D, H31_MOUSE, H3a, H3g, H3.1-291, H3h, H3.1-I, H3i, H32_MOUSE, H3-clustered histone 2, H3-clustered histone 3, H3-clustered histone 4, H3-clustered histone 6, H3-clustered histone 7, H3.2, H3b, H3f, H3.2-616, Hist2h3b, H3.2-615, Hist2h3c1, Hist2h3ca1, H3.2-614, Hist2h3c2, Hist2h3ca2, H31_RAT, |
| Phospho-Histone H3 (Ser10) Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 15 kDa |
| UniProt: | P68431 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | H3C1 |
| Application Verdünnung: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2µg/Test |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-Histone H3 (Ser10) antibody (orb106186), Dilution: 0.2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. |
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Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Histone H3 (Di Methyl K36) antibody, Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed. |
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Blank control: U-87MG. Primary Antibody (green line): Rabbit Anti-Phospho-Histone H3 antibody (orb106186), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Histone H3 (Ser10)) Polyclonal Antibody, Unconjugated (orb106186) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Histone H3 (Ser10)) Polyclonal Antibody, Unconjugated (orb106186) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Histone H3 (Ser10)) Polyclonal Antibody, Unconjugated (orb106186) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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