FGFR2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB10656
- Bilder (6)
| Artikelname: | FGFR2 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB10656 |
| Hersteller Artikelnummer: | orb10656 |
| Alternativnummer: | BYT-ORB10656-50,BYT-ORB10656-100,BYT-ORB10656-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human FGFR2 (21-120/821aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | BBDS, BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25, Fgfr-2, Fgfr-7, Fgfr2b, Fgfr7, KGFRTr, svs, FGFR2_HUMAN, FGFR2, K-sam (KGFR), Keratinocyte growth factor receptor, 2.7.10.1, KSAM, FGFR2_MOUSE, Keratinocyte growth factor receptor (KGFR), |
| FGFR2 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 89 kDa |
| UniProt: | P21802 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | FGFR2 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
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L6 cells were transfected with FGFR2, and stained with RABBIT ANTI-FGFR2 POLYCLONAL ANTIBODY, conjugated at 1:100 dilution. |
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Paraformaldehyde-fixed, paraffin embedded (human stomach cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FGFR2) Polyclonal Antibody, Unconjugated (orb10656) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human stomach cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FGFR2) Polyclonal Antibody, Unconjugated (orb10656) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FGFR2) Polyclonal Antibody, Unconjugated (orb10656) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: Lane 1: Mouse Spinal cord tissue lysates, Lane 2: Mouse NIH/3T3 cell lysates, Lane 3: Human 293T cell lysates, Lane 4: Human A431 cell lysates, Lane 5: Human HeLa cell lysates, Primary: Anti-FGFR2 (orb10656) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 89 kDa, Observed band size: 142 kDa. |
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Tissue/Cell: MCF7, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (FGFR2) Polyclonal Antibody, Unconjugated (orb10656) 1:200, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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