LIS1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB10992
- Bilder (7)
| Artikelname: | LIS1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB10992 |
| Hersteller Artikelnummer: | orb10992 |
| Alternativnummer: | BYT-ORB10992-100,BYT-ORB10992-200,BYT-ORB10992-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human LIS1 (311-410/410aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | LIS1, LIS2, MDCR, MDS, NudF, PAFAH, LIS-1, MMS10-U, Mdsh, Ms10u, Pafaha, LIS1_BOVIN, PAFAH1B1, Lissencephaly-1 protein (LIS-1), PAF acetylhydrolase 45 kDa subunit (PAF-AH 45 kDa subunit), PAF-AH alpha (PAFAH alpha), LIS1_HUMAN, LIS1_MOUSE, LIS1_PIG, LIS1_RAT, |
| LIS1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 47 kDa |
| UniProt: | P43034 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | PAFAH1B1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LIS1) Polyclonal Antibody, Unconjugated (orb10992) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse thyroid gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (LIS1) Polyclonal Antibody, Unconjugated (orb10992) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (LIS1) Polyclonal Antibody, Unconjugated (orb10992) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LIS1) Polyclonal Antibody, Unconjugated (orb10992) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat thyroid gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (LIS1) Polyclonal Antibody, Unconjugated (orb10992) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Hippocampus (Mouse) Lysate at 40 ug, Primary: Anti-LIS1 (orb10992) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 49 kD. |
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Tissue/Cell: Mouse embryos, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-LIS1 Polyclonal Antibody, Unconjugated (orb10992) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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