Laminin subunit beta-1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB10997
- Bilder (7)
| Artikelname: | Laminin subunit beta-1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB10997 |
| Hersteller Artikelnummer: | orb10997 |
| Alternativnummer: | BYT-ORB10997-100,BYT-ORB10997-200,BYT-ORB10997-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Laminin subunit beta-1 (901-1000/1786aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | CLM, LIS5, D130003D08Rik, Lamb-1, Lamb1-1, LAMB1_HUMAN, LAMB1, Laminin B1 chain, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, LAMB1_MOUSE, D3ZQN7_RAT, |
| Laminin subunit beta-1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 198 kDa |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | LAMB1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
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Paraformaldehyde-fixed, paraffin embedded (human kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (orb10997) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (orb10997) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (orb10997) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: Lane 1: Heart (Mouse) Lysate at 40 ug, Lane 2: Skin (Mouse) Lysate at 40 ug, Lane 3: Placenta (Mouse) Lysate at 40 ug, Lane 4: U2os (Human) Cell Lysate at 30 ug, Lane 5: Huvec (Human) Cell Lysate at 30 ug, Lane 6: HepG2 (Human) Cell Lysate at 30 ug, Lane 7: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 8: A549 (Human) Cell Lysate at 30 ug, Lane 9: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-laminin (orb10997) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 220-240 kD, Observed band size: 240 kD. |
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Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-laminin Polyclonal Antibody, Unconjugated (orb10997) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: rat intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-laminin Polyclonal Antibody, Unconjugated (orb10997) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: Transplantation tumor of In nude mice, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-laminin Polyclonal Antibody, Unconjugated (orb10997) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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