PAR1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB11234
- Bilder (7)
| Artikelname: | PAR1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB11234 |
| Hersteller Artikelnummer: | orb11234 |
| Alternativnummer: | BYT-ORB11234-50,BYT-ORB11234-100,BYT-ORB11234-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human PAR1 (251-350/425aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | CF2R, HTR, PAR-1, PAR1, TR, PAR1_PAPHA, F2R, Thrombin receptor, BTHR12, PAR1_HUMAN, Coagulation factor II receptor, |
| PAR1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 42 kDa |
| UniProt: | P25116 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | F2R |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Thrombin receptor/ PAR1) Polyclonal Antibody, Unconjugated (orb11234) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Thrombin receptor PAR1) Polyclonal Antibody, Unconjugated (orb11234) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
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Sample: Brain (Rat) Lysate at 30 ug, Liver (Mouse) Lysate at 30 ug, Primary: Anti-PAR-1 (orb11234) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 42kD, Observed band size: 44kD. |
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Sample: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-PAR1 (orb11234) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD. |
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Sample: Lane 1: U2os (Human) Cell Lysate at 30 ug, Lane 2: U251 (Human) Cell Lysate at 30 ug, Lane 3: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 4: K562 (Human) Cell Lysate at 30 ug, Lane 5: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-Thrombin receptor (orb11234) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 62 kD. |
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Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated (orb11234) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: human mammary cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated (orb11234) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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