FOXO1A Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB1124
- Bilder (6)
| Artikelname: | FOXO1A Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB1124 |
| Hersteller Artikelnummer: | orb1124 |
| Alternativnummer: | BYT-ORB1124-50,BYT-ORB1124-100,BYT-ORB1124-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human FKHR (165-270/655aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | FKH1, FKHR, FOXO1A, Afxh, Fkhr1, FOXO1_HUMAN, FOXO1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1_MOUSE, FOXO1_RAT, |
| FOXO1A Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 72 kDa |
| UniProt: | Q12778 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | FOXO1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test |
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Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-FOXO1A antibody (orb1124), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-FOXO1A antibody (orb1124), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FOXO1A) Polyclonal Antibody, Unconjugated (orb1124) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-FOXO1A (orb1124) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 72 kD, Observed band size: 72 kD. |
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Sample: Lane 1: Lymph node (Rat) Lysate at 40 ug, Lane 2: Bone (Rat) Lysate at 40 ug, Lane 3: Liver (Rat) Lysate at 40 ug, Primary: Anti-FOXO1A (orb1124) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 78 kD, Observed band size: 75 kD. |
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Tissue/Cell: human bladder carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-FOXO1A Polyclonal Antibody, Unconjugated (orb1124) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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