GPLD1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB1126
Artikelname: GPLD1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB1126
Hersteller Artikelnummer: orb1126
Alternativnummer: BYT-ORB1126-50,BYT-ORB1126-100,BYT-ORB1126-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human GPLD1/GPI-PLD (51-150/840aa)
Konjugation: Unconjugated
Alternative Synonym: GPIPLD, GPIPLDM, PIGPLD, PIGPLD1, PLD, 6330541J12Rik, PHLD_HUMAN, GPLD1, PI-G PLD, Glycoprotein phospholipase D, Glycosyl-phosphatidylinositol-specific phospholipase D (GPI-PLD | GPI-specific phospholipase D), 3.1.4.50, PHLD_MOUSE, PHLD_RAT,
GPLD1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 90 kDa
UniProt: P80108
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: GPLD1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200
Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GPLD1) Polyclonal Antibody, Unconjugated (orb1126) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GPLD1) Polyclonal Antibody, Unconjugated (orb1126) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GPLD1) Polyclonal Antibody, Unconjugated (orb1126) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GPLD1) Polyclonal Antibody, Unconjugated (orb1126) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Human K562 cell lysates, Lane 2: Human HepG2 cell lysates, Primary: Anti-GPLD1 (orb1126) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90 kDa, Observed band size: 100 kDa.



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