AKT2 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB11297
Artikelname: AKT2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB11297
Hersteller Artikelnummer: orb11297
Alternativnummer: BYT-ORB11297-50,BYT-ORB11297-100,BYT-ORB11297-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human AKT2 (101-200/481aa)
Konjugation: Unconjugated
Alternative Synonym: HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA, 2410016A19Rik, PKB, AKT2_HUMAN, AKT2, Protein kinase Akt-2, Protein kinase B beta (PKB beta), RAC protein kinase beta (RAC-PK-beta), 2.7.11.1, AKT2_MOUSE, RAC-PK-beta, AKT2_RAT,
AKT2 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 56 kDa
UniProt: P31751
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: AKT2
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (AKT2) Polyclonal Antibody, Unconjugated (orb11297) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (AKT2) Polyclonal Antibody, Unconjugated (orb11297) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (AKT2) Polyclonal Antibody, Unconjugated (orb11297) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Cerebrum (mouse) Lysate at 40 ug, Primary: Anti-AKT2 (orb11297) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 56kD, Observed band size: 56kD.
Tissue/Cell: human laryngo carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-AKT2 Polyclonal Antibody, Unconjugated (orb11297) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



* Mehrwertsteuer und Versandkosten nicht enthalten.
Irrtümer und Preisänderungen vorbehalten
Verfügbarkeit
PER E-MAIL BESTELLEN
Produktgarantie und fachkundiger Support
BIOZOL