PTPN1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB11321
- Bilder (6)
| Artikelname: | PTPN1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB11321 |
| Hersteller Artikelnummer: | orb11321 |
| Alternativnummer: | BYT-ORB11321-50,BYT-ORB11321-100,BYT-ORB11321-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human PTP-1B (1-100/435aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | PTP1B, PTP-1B, PTP-HA2, Ptp, PTN1_HUMAN, PTPN1, Protein-tyrosine phosphatase 1B (PTP-1B), 3.1.3.48, PTN1_MOUSE, Protein-tyrosine phosphatase HA2 (PTP-HA2), PTN1_RAT, |
| PTPN1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 48 kDa |
| UniProt: | P18031 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | PTPN1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test |
|
|
Blank control (black line): HUVEC. Primary Antibody (green line): Rabbit Anti-PTP1B antibody (orb11321), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
|
|
Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (PTP1B) polyclonal Antibody, Unconjugated (orb11321) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
|
|
Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (PTP1B) polyclonal Antibody, Unconjugated (orb11321) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTP1B) Polyclonal Antibody, Unconjugated (orb11321) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Sample: Mcf-7 Cell Lysate at 40 ug, Primary: Anti-PTP1B (orb11321) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48kD, Observed band size: 48kD. |
|
|
Tissue/Cell: human endometrium tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-PTP-1B Polyclonal Antibody, Unconjugated (orb11321) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
Produktgarantie und fachkundiger Support
