SGCB Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB11363
Artikelname: SGCB Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB11363
Hersteller Artikelnummer: orb11363
Alternativnummer: BYT-ORB11363-50,BYT-ORB11363-100,BYT-ORB11363-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human SGCB (165-260/318aa)
Konjugation: Unconjugated
Alternative Synonym: A3b, LGMD2E, LGMDR4, SGC, SGCB_HUMAN, SGCB, Beta-SG, 43 kDa dystrophin-associated glycoprotein (43DAG),
SGCB Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 35 kDa
UniProt: Q16585
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: SGCB
Application Verdünnung: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SGCB) Polyclonal Antibody, Unconjugated (orb11363) at 1:200 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (human placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SGCB) Polyclonal Antibody, Unconjugated (orb11363) at 1:200 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SGCB) Polyclonal Antibody, Unconjugated (orb11363) at 1:200 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SGCB) Polyclonal Antibody, Unconjugated (orb11363) at 1:200 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SGCB) Polyclonal Antibody, Unconjugated (orb11363) at 1:200 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SGCB) Polyclonal Antibody, Unconjugated (orb11363) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.