SRC Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB11412
- Bilder (6)
| Artikelname: | SRC Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB11412 |
| Hersteller Artikelnummer: | orb11412 |
| Alternativnummer: | BYT-ORB11412-50,BYT-ORB11412-100,BYT-ORB11412-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human SRC (2-100/541aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ASV, SRC1, THC6, c-SRC, p60-Src, pp60c-src, SRC_HUMAN, SRC, Proto-oncogene c-Src, pp60c-src (p60-Src), 2.7.10.2, SRC_MOUSE, SRC_RAT, |
| SRC Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 61 kDa |
| UniProt: | P12931 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | SRC |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200-800 |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SRC) Polyclonal Antibody, Unconjugated (orb11412) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SRC) Polyclonal Antibody, Unconjugated (orb11412) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SRC) Polyclonal Antibody, Unconjugated (orb11412) at 1:400 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) for 90 minutes, and DAPI for nuclei staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SRC) Polyclonal Antibody, Unconjugated (orb11412) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-SRC (orb11412) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kD, Observed band size: 61 kD. |
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Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-SRC (orb11412) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kD, Observed band size: 61 kD. |
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