SREBP1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB11415
- Bilder (6)
| Artikelname: | SREBP1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB11415 |
| Hersteller Artikelnummer: | orb11415 |
| Alternativnummer: | BYT-ORB11415-50,BYT-ORB11415-100,BYT-ORB11415-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human SREBP-1 (301-450/1147aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | HMD, IFAP2, SREBP1, bHLHd1, ADD1, ADD-1, SREBP-1, SREBP-1c, SRBP1_HUMAN, SREBF1, Class D basic helix-loop-helix protein 1 (bHLHd1), Sterol regulatory element-binding transcription factor 1, SRBP1_MOUSE, SRBP1_RAT, Adipocyte determination- and differentiation-dependent factor 1 (ADD1), |
| SREBP1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 54/126 kDa |
| UniProt: | P36956 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | SREBF1 |
| Application Verdünnung: | IHC-P=1:200-500, IHC-F=1:200-500, IF=1:200-500 |
|
|
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (orb11415) at 1:200 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (orb11415) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (orb11415) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (orb11415) at 1:200 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (orb11415) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Sample: MCF-7 (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, SH-SY5Y (Human) Cell Lysate at 30 ug, U87MG (Human) Cell Lysate at 30 ug, A431 (Human) Cell Lysate at 30 ug, Primary: Anti-SREBP1 (orb11415) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 124/65 kD, Observed band size: 124 kD. |
Produktgarantie und fachkundiger Support
