TRIM21 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB11417
- Bilder (7)
| Artikelname: | TRIM21 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB11417 |
| Hersteller Artikelnummer: | orb11417 |
| Alternativnummer: | BYT-ORB11417-50,BYT-ORB11417-100,BYT-ORB11417-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human TRIM21 N-terminus (301-400/475aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | RNF81, RO52, Ro/SSA, SSA, SSA1, RO52_HUMAN, TRIM21, 52 kDa Ro protein, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, RING finger protein 81, Ro(SS-A), Sjoegren syndrome type A antigen (SS-A), Tripartite motif-containing protein 21, 2.3.2.27, |
| TRIM21 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 52 kDa |
| UniProt: | P19474 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | TRIM21 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
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Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TRIM21) Polyclonal Antibody, Unconjugated (orb11417) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TRIM21) Polyclonal Antibody, Unconjugated (orb11417) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TRIM21) Polyclonal Antibody, Unconjugated (orb11417) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TRIM21) Polyclonal Antibody, Unconjugated (orb11417) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-TRIM21 (orb11417) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD. |
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Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-TRIM21 (orb11417) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD. |
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Sample: U937 Cell Lysate at 40 ug, Primary: Anti-TRIM21 (orb11417) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 56 kD. |
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