TFF3 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB11465
- Bilder (6)
| Artikelname: | TFF3 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB11465 |
| Hersteller Artikelnummer: | orb11465 |
| Alternativnummer: | BYT-ORB11465-50,BYT-ORB11465-100,BYT-ORB11465-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P |
| Spezies Reaktivität: | Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human TFF3 (51-80/94aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ITF, P1B, TFI, TFF3_HUMAN, TFF3, Intestinal trefoil factor (hITF), Polypeptide P1.B (hP1.B), |
| TFF3 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 6.5 kDa |
| UniProt: | Q07654 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | TFF3 |
| Application Verdünnung: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (orb11465) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (orb11465) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (orb11465) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (orb11465) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (orb11465) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Tissue/Cell: rat colon tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TFF3 Polyclonal Antibody, Unconjugated (orb11465) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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