Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.6 kDa. The protein migrates as 40-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
Immobilized Biotinylated Human IGF-II, Avitag, Fc Tag at 2 µg/mL (100 µL/well) on streptavidin (Cat. No. orb668907) precoated (1 µg/well) plate can bind Human IGFBP-3, His Tag (Cat. No. orb1146966) with a linear range of 0.01-0.313 µg/mL (QC tested).
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