Application Notes: This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 63.2 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
Immobilized Human IL-5, His Tag (Cat. No. orb612148) at 5 µg/mL (100 µL/well) can bind Human IL-5 R alpha, Fc Tag (Cat. No. orb1146968) with a linear range of 0.6-10 ng/mL (QC tested).
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