Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.9 kDa. The protein migrates as 22-24 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
Immobilized Human IL-4, His Tag (Cat. No. orb1149283) at 5 µg/mL (100 µL/well) can bind Human IL-4 R alpha, Fc Tag (Cat. No. orb545738) with a linear range of 0.5-16 ng/mL (QC tested).
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