TRADD Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB11504
Artikelname: TRADD Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB11504
Hersteller Artikelnummer: orb11504
Alternativnummer: BYT-ORB11504-50,BYT-ORB11504-100,BYT-ORB11504-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from the middle of mouse TRADD (111-230/310aa)
Konjugation: Unconjugated
Alternative Synonym: Hs.89862, 9130005N23Rik, TRADD_HUMAN, TRADD, TNFR1-associated DEATH domain protein, TNFRSF1A-associated via death domain, TRADD_MOUSE,
TRADD Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 34 kDa
UniProt: Q3U0V2
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: TRADD
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-TRADD (orb11504) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.
Sample: Jurkat (Human) Cell Lysate at 30 ug, MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-TRADD (orb11504) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.
Sample: Lane 1: Colon carcinoma (Human) Lysate at 30 ug, Lane 2: Brain (Mouse) Lysate at 30 ug, Primary: Anti-TRADD (orb11504) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 35kD, Observed band size: 34kD.
Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TRADD Polyclonal Antibody, Unconjugated (orb11504) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat pancreas tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TRADD Polyclonal Antibody, Unconjugated (orb11504) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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