Trk A + B + C Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB11516
- Bilder (5)
| Artikelname: | Trk A + B + C Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB11516 |
| Hersteller Artikelnummer: | orb11516 |
| Alternativnummer: | BYT-ORB11516-50,BYT-ORB11516-100,BYT-ORB11516-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Trk A (668-750/821aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA, Tkr, NTRK1_HUMAN, NTRK1, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A (Trk-A), gp140trk, 2.7.10.1, NTRK1_MOUSE, NTRK1_RAT, Slow nerve growth factor receptor, p140-TrkA (Trk-A), |
| Trk A + B + C Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 90 kDa |
| UniProt: | P04629 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | NTRK1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Trk A + B + C) Polyclonal Antibody, Unconjugated (orb11516) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 3: Large intestine (Mouse) Lysate at 40 ug, Lane 4: Uterus (Mouse) Lysate at 40 ug, Primary: Anti-Trk A + B + C (orb11516) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 140/110 kD, Observed band size: 120 kD. |
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Sample: Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 2: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-Trk A + B + C (orb11516) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD. |
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Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Trk A/B/C Polyclonal Antibody, Unconjugated (orb11516) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Trk A/B/C Polyclonal Antibody, Unconjugated (orb11516) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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