0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Application Verdünnung:
Immunohistochemistry (FFPE): 1-2ug/ml
Anwendungsbeschreibung:
Application Notes: Optimal dilution of the CD2 antibody should be determined by the researcher
IHC staining of FFPE human lymph node tissue with CD2 antibody (clone LFA2/7102) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with C2 antibody (clone LFA2/7102) at 2 ug/ml in PBS for 30 min RT. Strong cell surface staining observed. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with CD2 antibody (clone LFA2/7102). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD2 antibody (clone LFA2/7102) as confirmation of integrity and purity.
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CD2 antibody (clone LFA2/7102). These results demonstrate the foremost specificity of the LFA2/7102 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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