Application Notes: Optimal dilution of the CD38 antibody should be determined by the researcher
Western blot testing of human 1) spleen and 2) Raji cell lysate using CD38 antibody (clone CD384328). Expected molecular weight: 34-46 kDa depending on glycosylation level.
Western blot testing of human Raji cell lysate using CD38 antibody (clone CD38/4328). Expected molecular weight: 34-46 kDa depending on glycosylation level.
IHC staining of FFPE human tonsil tissue with CD38 antibody (clone CD38/4328) at 2 ug/ml in PBS for 30 min RT. Strong cytoplasmic and membranous staining is observed. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma tissue with CD38 antibody (clone CD38/4328) at 2 ug/ml in PBS for 30 min RT. Strong cytoplasmic and membranous staining observed. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human cerebral cortex tissue with CD38 antibody (clone CD38/4328) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CD38 antibody (clone CD38/4328). These results demonstrate the foremost specificity of the CD38/4328 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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