Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.3 kDa. The protein migrates as 32-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
Human NRP1, Fc Tag (Cat. No. orb257718) captured on Protein A Chip can bind Human VEGF-B, His Tag (Cat. No. orb1152444) with an affinity constant of 23.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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