Visfatin Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB11562
- Bilder (6)
| Artikelname: | Visfatin Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB11562 |
| Hersteller Artikelnummer: | orb11562 |
| Alternativnummer: | BYT-ORB11562-50,BYT-ORB11562-100,BYT-ORB11562-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, IF, IHC-Fr, IHC-P |
| Spezies Reaktivität: | Human, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human PBEF (301-400/491aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | 1110035O14Rik, PBEF, PBEF1, VF, VISFATIN, NAmPRTase, NAMPT_HUMAN, NAMPT, Pre-B-cell colony-enhancing factor 1 (Pre-B cell-enhancing factor), 2.4.2.12, NAMPT_MOUSE, Pre-B-cell colony-enhancing factor 1 homolog (PBEF), NAMPT_RAT, |
| Visfatin Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 55 kDa |
| UniProt: | P43490 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | NAMPT |
| Application Verdünnung: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test |
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Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-Visfatin antibody (orb11562), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Visfatin) Polyclonal Antibody, Unconjugated (orb11562) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Visfatin (orb11562) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD. |
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Sample: Lane 1: Brain (Rat) Lysate at 30 ug, Lane 2: Liver (Rat) Lysate at 30 ug, Primary: Anti-VISFATIN-1 (orb11562) at 1:200 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:3000 dilution, Predicted band size: 55kD, Observed band size: 54kD. |
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Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Visfatin Polyclonal Antibody, Unconjugated (orb11562) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: Rat lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Visfatin Polyclonal Antibody, Unconjugated (orb11562) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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