WWOX Recombinant Rabbit Monoclonal Antibody, Clone: [F11B6], Unconjugated

Artikelnummer: BYT-ORB1172795
Artikelname: WWOX Recombinant Rabbit Monoclonal Antibody, Clone: [F11B6], Unconjugated
Artikelnummer: BYT-ORB1172795
Hersteller Artikelnummer: orb1172795
Alternativnummer: BYT-ORB1172795-25,BYT-ORB1172795-50,BYT-ORB1172795-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: FC, ICC, IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Rat
Konjugation: Unconjugated
Alternative Synonym: D16S432E, DEE28, EIEE28, FOR, FRA16D, HHCMA56, PRO0128, SCAR12, SDR41C1, WOX1, 5330426P09Rik, 9030416C10Rik, WWOX_HUMAN, WWOX, Fragile site FRA16D oxidoreductase, Short chain dehydrogenase/reductase family 41C member 1, 1.1.1.-, WWOX_MOUSE,
WWOX Recombinant Rabbit Monoclonal Antibody
Klonalität: Recombinant
Konzentration: 1mg/ml
Klon-Bezeichnung: [F11B6]
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: WWOX
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: MCF-7, 2: Mouse ovary, 3: Rat brain, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 47 kDa, Observed MW: 47 kDa.
Cell line: A549, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1172795.
Cell line: MCF-7, Fixation: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary Ab Dilution: 1:50, Secondary Ab: Goat Anti-Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1172795.
Tissue: Human cerebrum, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 4C overnight, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172795.
Tissue: Human testis, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172795.
Tissue: Rat cerebrum, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172795.