Mono-Methyl-Histone H4 (Lys12) Recombinant Rabbit Monoclonal Antibody, Clone: [B11E4], Unconjugated
Artikelnummer:
BYT-ORB1172948
- Bilder (6)
| Artikelname: | Mono-Methyl-Histone H4 (Lys12) Recombinant Rabbit Monoclonal Antibody, Clone: [B11E4], Unconjugated |
| Artikelnummer: | BYT-ORB1172948 |
| Hersteller Artikelnummer: | orb1172948 |
| Alternativnummer: | BYT-ORB1172948-25,BYT-ORB1172948-50,BYT-ORB1172948-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H4C1 | Histone H4 (me1-K12), me-Histone H4, methyl-Histone H4, H4K12me1, me-H4, H4-16, H4/p, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, HIST4H4, H4/o, H4C16, HIST2H4B, H4/m, H4FM, H4M, HIST1H4I, TEBIVANED4, TEVANED4, H4FA, HIST1H4A, H4/b, H4FB, HIST1H4D, dJ221C16.9, H4, H4/c, H4FC, HIST1H4F, H4/d, H4F2iii, H4FD, HIST1H4K, dJ160A22.1, H4/e, H4F2iv, H4FE, HIST1H4J, TEBIVANED2, TEVANED2, dJ160A22.2, H4/g, H4FG, HIST1H4C, TEBIVANED1, TEVANED1, dJ221C16.1, H4/h, H4FH, HIST1H4H, H4/I, H4FI, HIST1H4B, H4/j, H4FJ, HIST1H4E, TEBIVANED3, TEVANED3, H4.k, H4/k, H4FK, HIST1H4L, FO108, H4/n, H4F2, H4FN, HIST2H4, HIST2H4A, Gm11275, H4f16, Hist1h4m, Hist1h4n, B130044J01Rik, 1700024H08Rik, H2c8, H4_DROME, His4, His4r, His4:CG31611, His4:CG33869, His4:CG33871, His4:CG33873, His4:CG33875, His4:CG33877, His4:CG33879, His4:CG33881, His4:CG33883, His4:CG33885, His4:CG33887, His4:CG33889, His4:CG33891, His4:CG33893, His4:CG33895, His4:CG33897, His4:CG33899, His4:CG33901, His4:CG33903, His4:CG33905, His4:CG33907, His4:CG33909, H4r, H4_HUMAN, H4/A, H4FO, H4_MOUSE, H4-53, H4-12, H4_SCHPO, hhf1, hhf2, hhf3, h4.1, h4.2, h4.3, |
| Mono-Methyl-Histone H4 (Lys12) Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [B11E4] |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | H4C1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100 |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1. HeLa, 2. NIH-3T3, 3. BRL, 4. Mouse heart, 5. Rat heart, 6. Mouse kidney, 7. Rat kidney, 8. Recombinant Histone H4, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 11 kDa, Observed MW: 11 kDa. |
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Cell line: HeLa, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1172948. |
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Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1172948. |
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Tissue: Human prostate hyperplasis, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab Dilution: 1:500, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172948. |
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Tissue: Mouse liver, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab Dilution: 1:500, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172948. |
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Tissue: Rat stomach, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab Dilution: 1:500, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172948. |
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