PTPN2 Recombinant Mouse Monoclonal Antibody, Clone: [F3H2], Unconjugated

Artikelnummer: BYT-ORB1173018
Artikelname: PTPN2 Recombinant Mouse Monoclonal Antibody, Clone: [F3H2], Unconjugated
Artikelnummer: BYT-ORB1173018
Hersteller Artikelnummer: orb1173018
Alternativnummer: BYT-ORB1173018-25,BYT-ORB1173018-50,BYT-ORB1173018-100
Hersteller: Biorbyt
Wirt: Mouse
Kategorie: Antikörper
Applikation: FC, IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Konjugation: Unconjugated
Alternative Synonym: PTN2, PTPT, TC-PTP, TC45, TC48, TCELLPTP, TCPTP, PTN2_HUMAN, PTPN2, T-cell protein-tyrosine phosphatase (TCPTP), 3.1.3.48, PTN2_MOUSE, Protein-tyrosine phosphatase PTP-2, MPTP, PTN2_RAT, Protein-tyrosine phosphatase PTP-S,
PTPN2 Recombinant Mouse Monoclonal Antibody
Klonalität: Recombinant
Konzentration: 1mg/ml
Klon-Bezeichnung: [F3H2]
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: PTPN2
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100, Flow-Cyt=1:50-100
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:5000, Primary ab incubation condition: room temperature 2h, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HepG2, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 45-50 kDa, Observed MW: 45-50 kDa.
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:5000, Primary ab incubation condition: room temperature 2h, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HL-60, A20, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 45-50 kDa, Observed MW: 45-50 kDa.
Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Secondary ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1173018.
Tissue: Human breast cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173018.
Tissue: Mouse liver, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173018.
Tissue: Rat stomach, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173018.