Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.7 kDa. The protein migrates as 22-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human TSLP (R127A, R130A), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human TSLP R, Fc Tag at 5 ug/ml (100 uL/well) can bind Human TSLP (R127A, R130A), His Tag with a linear range of 0.8-6 ng/mL.
Immobilized Human TSLP (R127A, R130A), His Tag at 1 ug/ml (100 uL/well) can bind Anti-TSLP, Human IgG2 with a linear range of 0.4-3 ng/mL.
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