Application Notes: This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 46.9 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human TNFSF11, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin at 0.5 ug/ml (100 uL/well) can bind Human TNFSF11, Fc Tag with a linear range of 0.1-3 ng/mL.
Immobilized Human OPG, His Tag at 10 ug/ml (100 uL/well) can bind Human TNFSF11, Fc Tag with a linear range of 0.3-2 ng/mL.
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