Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.0 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Cynomolgus SIRP alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Cynomolgus/Rhesus macaque CD47, Fc Tag at 2 ug/ml (100 uL/well) can bind Cynomolgus SIRP alpha, His Tag with a linear range of 0.313-2.5 ug/ml.
Immobilized Biotinylated Human CD47, Fc, Avitag at 1 ug/ml (100 uL/well) on streptavidin precoated (0.2ug/well) plate, can bind Cynomolgus SIRP alpha, His Tag with a linear range of 0.039-0.625 ug/ml.
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