Application Notes: This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 737-RKRR-740, and will be partially processed into N (alpha chain) and C-terminal fragment (partial beta chain) with calculated MW of 81.0 kDa and
Human IGF-I R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human IGF-I R, His Tag at 5 ug/ml (100 uL/well) can bind Human IGF-I, Fc Tag with a linear range of 0.04-0.313 ug/ml.
Immobilized Human IGF-I R, His Tag at 5 ug/ml (100 uL/well) can bind Biotinylated Human IGF-II, Avitag, Fc Tag with a linear range of 0.156-5 ug/ml.
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