Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 48.5 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human B7-H3 (4Ig), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human B7-H3 (4Ig), His Tag at 2 ug/ml (100 uL/well) can bind Monoclonal Anti-Human B7-H3/B7-H3 (4Ig) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL.
Immobilized Monoclonal Anti-Human B7-H3/B7-H3 (4Ig) Antibody, Human IgG1 at 2 ug/ml (100 uL/well) can bind Human B7-H3 (4Ig), His Tag with a linear range of 1-10 ng/mL.
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