Application Notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 20.5 kDa. The protein migrates as 23 kDa and 26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human GITR Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human GITR Ligand, His Tag at 2 ug/ml (100 uL/well) can bind Human GITR, Fc Tag with a linear range of 0.1-4 ng/mL.
Immobilized Human GITR Ligand, His Tag at 2 ug/ml (100 uL/well) can bind Human GITR, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.2-8 ng/mL.
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