Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung:
Liquid
Target-Kategorie:
OGT
Anwendungsbeschreibung:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 110kDa band observed in Rat Pancreas lysates calculated MW of 116kDa according to NP_858058.2). An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.05-0.2µg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/mL.Immunofluorescence: Strong expression of the protein seen in the nucleus of HeLa, U2OS and Glioblastoma U251 cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10µg/mL
orb1247175 (0.05 ug/ml) staining of Rat Pancreas lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1247175 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and membrane/cytoplasmic staining.
orb1247175 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).
orb1247175 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining.
orb1247175 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1247175 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining.
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