Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung:
Liquid
Target-Kategorie:
CAV1
Anwendungsbeschreibung:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 22kDa band observed in lysates of cell lines A431 and A549 and in Human Heart lysates (calculated MW of 20.5kDa according to NP_001744.2). Recommended concentration: 0.001-0.01µg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Pig Heart and Spleen, and Mouse and Rat Lung for this particular batch.Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of A431 cells and in the cytoplasm and Golgi of U2OS cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10µg/mL
orb1248004 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
orb1248004 (0.003 ug/ml) staining of A431 (A) and (0.001 ug/ml) A549 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248004 (0.003 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248004 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi and membrane staining.
orb1248004 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi and cytoplasmic staining.
orb1248004 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).
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