Western blot analysis of extracts of various cell lines, using HHLA2 antibody (orb1254914) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human colon using HHLA2 antibody (orb1254914) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using HHLA2 antibody (orb1254914) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HHLA2 antibody (orb1254914) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using HHLA2 antibody (orb1254914) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using HHLA2 antibody (orb1254914) at dilution of 1:100 (40x lens).
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