WNT9A Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1256081
Artikelname: WNT9A Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1256081
Hersteller Artikelnummer: orb1256081
Alternativnummer: BYT-ORB1256081-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 206-365 of human WNT9A (NP_003386.1).
Konjugation: Unconjugated
Alternative Synonym: Protein Wnt-9a, Protein Wnt-14, WNT9A, WNT14
WNT9A Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 30kDa
UniProt: O14904
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: WNT9A
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of mouse lung, using WNT9A antibody (orb1256081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using WNT9A antibody (orb1256081) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using WNT9A antibody (orb1256081) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using WNT9A antibody (orb1256081) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using WNT9A antibody (orb1256081) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using WNT9A antibody (orb1256081) at dilution of 1:100. Blue: DAPI for nuclear staining.